How do I choose particle size and pore size in HPLC columns?

3µm and 5µm particles are common for analytical work. 100Å pores are standard for small molecules and 300Å pores are suitable for peptides and proteins.

What are equivalent or alternate HPLC columns?

Equivalent HPLC columns are replacement columns that match branded stationary phase chemistry and dimensions to allow seamless method transfer.

What is a ghost trapping column in HPLC?

Ghost trapping columns remove background impurities and solvent contaminants to improve baseline stability and reduce ghost peaks.

Itolsep HPLC Columns

Q: What is an HPLC column and how does it work?

An HPLC column is the primary separation device in high performance liquid chromatography. It contains a stationary phase that separates compounds based on chemical interactions.

Q: What is the difference between C18, C8, Phenyl and HILIC columns?

C18 columns provide strong hydrophobic retention. C8 offers moderate retention. Phenyl columns allow aromatic selectivity. HILIC columns are used for polar compounds.

Complete Itolsep HPLC column listing. Use search to filter by dimensions, particle size, and specifications.

Description	Part Number	Phase	Length (mm)	ID (mm)	Particle (µm)	Pore (Å)
ItolSep ES Carbohydrate 5μm 100Å 150 X 4.6mm	IC553-CARB	Itolsep		4.6	5	100
ItolSep ES Carbohydrate 5μm 100Å 250 X 4.6mm	IE553-CARB	Itolsep		4.6	5	100
ItolSep CAG Ag+ 4% 9μm 50mmX7.8mm	IA66A-CAG	Itolsep	50	50	9
ItolSep CAG Ag+ 4% 9μm 100mmX7.8mm	IB66A-CAG	Itolsep	100	100	9
ItolSep CAG Ag+ 4% 9μm 150mmX7.8mm	IC66A-CAG	Itolsep	150	150	9
ItolSep CAG Ag+ 4% 9μm 300mmX7.8mm	IW66A-CAG	Itolsep	300	300	9
ItolSep CNM Na+ 4% 8μm 50mmX7.8mm	IA66A-CNM	Itolsep	50	50	8
ItolSep CNM Na+ 4% 8μm 100mmX7.8mm	IB66A-CNM	Itolsep	100	100	8
ItolSep CNM Na+ 4% 8μm 150mmX7.8mm	IC66A-CNM	Itolsep	150	150	8
ItolSep CNM Na+ 4% 8μm 300mmX7.8mm	IW66A-CNM	Itolsep	300	300	8
ItolSep CNM Na+ 4% 9μm 300mmX7.8mm	IW6NA-CNM	Itolsep	300	300	9
ItolSep CNM Na+ 4% 8μm 50mmX4.6mm	IA56A-CNM	Itolsep	50	50	8
ItolSep CNM Na+ 4% 8μm 150mmX4.6mm	IC56A-CNM	Itolsep	150	150	8
ItolSep CNM Na+ 4% 8μm 250mmX4.6mm	IB56A-CNM	Itolsep	250	250	8
ItolSep CAM Ca2+ 8% 8μm 50mmX7.8mm	IA66A-CAM	Itolsep	50	50	8
ItolSep CAM Ca2+ 8% 8μm 100mmX7.8mm	IB66A-CAM	Itolsep	100	100	8
ItolSep CAM Ca2+ 8% 8μm 150mmX7.8mm	IC66A-CAM	Itolsep	150	150	8
ItolSep CAM Ca2+ 8% 8μm 300mmX7.8mm	IW66A-CAM	Itolsep	300	300	8
ItolSep CAM Ca2+ 8% 9μm 300mmX7.8mm	IW6NA-CAM	Itolsep	300	300	9
ItolSep CAM Ca2+ 8% 6μm 300mmX8.0mm	IWESA-CAM	Itolsep	300	300	6
ItolSep CAM Ca2+ 8% 8μm 50mmX4.6mm	IA56A-CAM	Itolsep	50	50	8
ItolSep CAM Ca2+ 8% 8μm 150mmX4.6mm	IC56A-CAM	Itolsep	150	150	8
ItolSep CAM Ca2+ 8% 8μm 250mmX4.6mm	IE56A-CAM	Itolsep	250	250	8
ItolSep CPM Pb2+ 8% 8μm 50mmX7.8mm	IA66A-CPM	Itolsep	50	50	8
ItolSep CPM Pb2+ 8% 8μm 100mmX7.8mm	IB66A-CPM	Itolsep	100	100	8
ItolSep CPM Pb2+ 8% 8μm 150mmX7.8mm	IC66A-CPM	Itolsep	150	150	8
ItolSep CPM Pb2+ 8% 8μm 300mmX7.8mm	IW66A-CPM	Itolsep	300	300	8
ItolSep COA Monosaccharide H+ 8% 8μm 50mmX7.8mm	IA66A-COA	Itolsep	50	50	8
ItolSep COA Monosaccharide H+ 8% 8μm 100mmX7.8mm	IB66A-COA	Itolsep	100	100	8
ItolSep COA Monosaccharide H+ 8% 8μm 150mm x 7.8 mm	IC66A-COA	Itolsep	150	150	8
ItolSep COA Monosaccharide H+ 8% 8μm 300mm x 7.8 mm	IW66A-COA	Itolsep	300	300	8
ItolSep COA Monosaccharide H+ 8% 8μm 250mm x 4.6 mm	IE56A-COA	Itolsep	250	250	8
ItolSep Carbohydrate 5μm 100A 150 X 4.6mm	IC553-CARB	Itolsep		4.6	5
ItolSep Carbohydrate 5μm 100A 250 X 4.6mm	IE553-CARB	Itolsep		4.6	5

Frequently Asked Questions (FAQs) – HPLC Columns

What is an HPLC column and how does it work?

An HPLC column is the core component of a High Performance Liquid Chromatography system. It contains a stationary phase that separates analytes based on polarity, hydrophobicity, ion-exchange properties, or molecular size.

What is the difference between C18, C8, Phenyl and HILIC columns?

C18 columns are reversed-phase columns with strong hydrophobic retention. C8 offers shorter retention. Phenyl columns provide π-π interaction selectivity. HILIC columns are used for polar compounds and hydrophilic interaction chromatography.

How do I select the correct particle size and pore size?

3µm and 5µm particles are common for analytical HPLC. Smaller particles provide higher efficiency but increase backpressure. 100Å pores are standard for small molecules, while 300Å pores are suitable for peptides and proteins.

What are alternate or equivalent HPLC columns?

Alternate HPLC columns are replacement columns designed to match the stationary phase chemistry, dimensions, and performance characteristics of branded columns for seamless method transfer.

What are Ghost Trapping Columns used for?

Ghost trapping columns remove system impurities and solvent contaminants that cause ghost peaks, improving baseline stability and analytical accuracy.

Quick Expert Answers

Best HPLC column for pharmaceutical analysis: C18 reversed phase columns are widely used due to stability and reproducibility.

Best column for polar compounds: HILIC columns provide strong retention for hydrophilic molecules.

How to reduce ghost peaks? Use Ghost Trapping Columns and high purity solvents.

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